WDF Dye bilong Sysmex 5-Part Hematology Analyzers em i wanpela spesel 'fluorescent reagent' we ol i wokim bilong 'automated optical flow cytometry', we i save go insait long ol 'permeabilized white blood cell membranes' bilong pasim ol 'cellular nucleic acids' bilong 'differential cell counting' na 'scattergram separation'.
Wanem em ol Hematology Stain / Dye Reagents?
Ol 'automated hematology dye reagents' em ol spesel 'fluorophore' solusen we ol i wokim bilong ol 'optical detection channels' insait long ol 'multi-parameter cell counters'. I no olsem ol 'manual blood smear stains' we ol i save yusim long 'conventional microscopy', ol dispela 'reagents' i gat ol 'synthetic fluorescent compounds' we i stap insait long ol 'organic solvent carriers'. Long taim bilong redim ol sel long masin, dispela samting i save go insait long ol sel we ol i bin kilim na pasim RNA na DNA insait long sel, na kamapim ol spesifik 'fluorescence signal' aninit long 'laser excitation'.
Rol long Sel Klasifikesen / Diteksen
Ol 'fluorescent dye reagents' i save helpim long luksave long planti sel long rot bilong skelim lait i stap nabaut na strong bilong 'fluorescence' insait long 'flow cell'.
Nucleic Acid Binding:Fluorophore i save pas long RNA na DNA insait long sel, na i save givim lait strong we i stret wantaim 'cellular nucleic acid content' na 'metabolic activity'.
Leukocyte Differential:Em i brukim ol bikpela limfosait, monosait, nutrofil, na eosinofil i go long ol narapela narapela grup long tupela-dimensenel skatagram.
Abnormal Cell Flagging:I luksave long ol 'atypical lymphocytes' na ol 'immature cells' bihainim ol 'elevated fluorescent signals' we i kam long ol bikpela RNA na DNA konsentresen.
Diteksen Senel
Ol masin bilong skelim 'hematology' we i gat 5-pela hap bilong en i save yusim ol 'discrete fluorescent reaction channels' long prosesim ol spesifik sel populesen:
WDF Channel:I skelim namba bilong ol waitpela sel bilong blut, ol 'atypical lymphocytes', na ol 'immature granulocytes' yusim 'side fluorescence' na 'side scatter'.
WNR Senel:Skelim namba bilong ol waitpela sel bilong blut na ol retpela sel bilong blut we i gat 'nucleated' long rot bilong rausim ol 'nuclear fluorescence signals'.
WPC Channel:Ol 'screen' bilong ol 'precursor cells', 'abnormal lymphocytes', na ol 'progenitor cell' populesen.
RET Senel:Em i skelim ol 'reticulocytes' na 'mature erythrocytes' long rot bilong makim 'intracellular ribosomal RNA'.
PLT Senel:I givim ol 'optical platelet counts' we i yusim ol 'fluorescent markers' long stopim ol 'cell fragments' o 'microcytic erythrocytes' long bagarapim.
Compatible Analyzer Series
Ol masin bilong skelim blut i gat 5-pela hap:Ol 'modular high-throughput platforms, ol 'standalone floor-standing units, na ol liklik benchtop sistem.
Ol Prodak Aidentifaia i wankain:STAC-DYE-SYSWNR, STAC-DYE-SYSWDF, STAC-DYE-SYSWPC, STAC-DYE-SYSRET, STAC-}SYSRET{9}}SYSRET
Ol spesifikasen bilong prodak
|
Paramita |
Spesifikasen |
|
Prodak Model |
STAC-DYE-SYSWDF |
|
Taget Senel |
WDF (White Blood Cell Differential) |
|
Active Molecule |
Polymethine Fluorescent Compound |
|
Eksaitesen / Emisen Wevlengt |
633–635 nm / 650–670 nm |
|
pH mak |
7.00-7.40 long 25 digri |
|
Stretpela (CV) |
WBC Liklik o wankain olsem 3.0%, NEUT% Liklik o wankain olsem 5.0%, LYMPH% Liklik o wankain olsem 5.0% |
|
Korelesen ($R^2$) |
Bikpela o wankain olsem 0.99 |
|
Laip bilong putim long sel / Open Vial Stebiliti |
18 mun (i no op) / 60 de (i op) long 2 digri -35 digri |
Aplikesen na wok bilong 'reagent'
Routine 5-Pat Leukocyte Differential:Stainim ol 'intracellular nucleic acids' long brukim ol 'lymphocytes', 'monocytes', 'neutrophils', na 'eosinophils' i go insait long ol 'scattergram clusters'.
Immature Granulocyte Quantification:I kamapim ol narapela narapela 'fluorescent intensity signals' bilong ol 'precursor cells' long mekim ol i ken kaunim ol samting long masin na i no gat wok long glasim ol 'smear' long han.
Atypical Lymphocyte Detection:I luksave long ol RNA level we i go antap insait long ol 'reactive leukocytes' bilong kirapim ol 'automated analytical alert'.
Pakej / Stoa
Tempereja bilong putim:2 digri –35 digri . Putim long ol tudakpela kontena longwe long lait.
Ol samting bilong kontena:Ol bikpela -densiti polyethylene amber botol we i gat induksen-silim klos.
Ol samting bilong trenspot:I gat setifiket bilong karim ol kago long ples. Lukautim long taim bilong ais aninit long 0 digri.
Ol samting bilong 'hematology'
STAC-DYE-SYSWNR:Fluorescent dye reagent bilong skelim olgeta waitpela sel bilong blut na 'nucleated red blood cell analysis'.
STAC-DYE-SYSWPC:Hai-spesifisiti 'fluorescent stain reagent' bilong 'precursor cell' na 'abnormal leukocyte screening'.
STAC-DYE-SYSRET:'Fluorescent nucleic acid stain' bilong skelim 'reticulocyte fraction'.
STAC-DYE-SYSPLT:'Optical fluorescent dye reagent' bilong painimaut stret ol 'platelet' insait long ol kompleks sempel.
FAQ
Q: Olsem wanem WDF dai i save stopim signal ovalap namel long ol 'neutrophils' na 'eosinophils' long ol 'scattergrams'?
A: Dispela marasin i wok wantaim ol 'lyse reagents' we i save senisim ol 'cell membrane'. Dae i save go insait long nuklia DNA na saitoplasmik RNA, na kamapim ol narapela narapela sait fluoresens na sait skata intensiti kodineit bilong wanwan sel populesen.
Q: Wanem em i taim bilong stap gut bilong ol kontena bilong 'reagent' we i op?
A: Ol kontena we i op i save stap gut inap 60 de sapos ol i putim namel long 2 digri na 35 digri. Dispela 'solvent formulation' i save stopim 'active compound degradation' na 'solvent evaporation' aninit long ol standet 'laboratory humidity' kondisen.
Q: Ol dispela 'dye reagents' inap long putim insait long ol 'fluidic lines' na i no gat ol 'fluidic adjustments'?
A: Yes. Dispela 'reagent' i save holim wankain 'fluidic viscosity', 'laser absorption profiles', na ol 'operational parameters', na dispela i larim ol i ken konektim stret long ol 'flow channels' we i wankain.
Q: Olsem wanem ol i save sekim 'batch-to-batch fluorescent stability long taim bilong wokim ol samting?
A: Ol kontrol bilong 'liquid chromatography' i save sekim 'active molecule purity' bilong olgeta 'production batch', na bihain ol i save mekim 'multi-channel flow testing' long holim 'scattergram cluster positioning drift' insait long liklik o wankain olsem 2.0%.
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